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KMID : 0613820070170030345
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Journal of Life Science
2007 Volume.17 No. 3 p.345 ~ p.349
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Cloning, Purification, and Structural Characterization by 1D 1H-NMR of the PDZ domain of the Shank3 protein
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Sung Mee-Sook
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Abstract
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We wished to create a set of small molecular weight PDZ domain ligands that may be used in functional studies on the proteins AF6, PSD-95 and Shank. As a starting point, the Shank3 PDZ domain was cloned, purified, and characterized the structure of Shank3 PDZ domain by 1D 1H-NMR. Thechemical shift dispersion of the proton signals indicates that the purified Shank3 PDZ protein is very pure and globally well folded. Currently, we are working on improving the yield of the protein production for complete NMR structural analysis of the Shank3 PDZ domain.
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KEYWORD
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Shank3 PDZ, 1D 1H-NMR
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